One of the major challenges to culturing tissues remains the fabrication of industrialisable, complex, non-cytotoxic scaffolds, compatible with established cell culture protocols and characterisation methods.
Because all cells are different, PeptiGelDesign has commercialised a family of self-assembling hydrogels based on the fundamental understanding of small peptides in water. They mimic the cell microenvironment and provide a natural physiological environment for 3D cell culture.
PGD-Hydrogel is a peptide nanofiber 3D synthetic extra cellular matrix that promotes cell growth and migration. It utilizes specific synthetic peptides that, when at a concentration greater than the critical gelation concentration (CGC), and when presented with physiological level ionic strengths, self-assemble from fluid precursors into a hydrogel. Example areas of use include 3D cell culture, stem cell culture and directed differentiation.
We are the first to provide scientists with a scaffold solution that
- is ready to use
- does not suffer from batch to batch variation
- was demonstrated to support improved growth, migration and directed differentiation of various cell types such as
- stem cells (e.g., hMSCs, ASCs, IPSCs)
- endothelial cells
- epithelial cells
- does not interfere with cell staining
- is compatible with microscopy
- offers simple protocols for cell extraction and downstream mRNA, qPCR analysis as well as cell counting
- also qualifies for cell based assays
Our technology platform (easily functionalisable, of tuneable electrical net charge and with its mechanical properties), provides researchers with a unique control over the 3D architecture to meet their application′s specific needs, enabling hard and soft tissue models to be produced.
Translational (WO2014/044321), our products also have great potential for applications in the regenerative and medical field as they are animal free, biocompatible and biodegradable.
With unique shear thinning properties, our hydrogel technology platform can therefore potentially be used as primary packaging for the in-vivo delivery of drugs, cells or other biological factors. Non-immunogenic, our products are injectable, sprayable and are naturally muco-adhesive.
Our products are provided as formulated ready-to-use and easy-to-handle hydrogels for culture dishes, multi-well plates, or cell culture inserts. They can be used as substrates for 2D cell culture, as well as the encapsulation of cells for 3D culture.
We have demonstrated the reproducible printability and high viability of encapsulated cells retrieved from a patient and printed with a peptide hydrogel matrix.